TÉCNICAS
ANALYTICAL TECHNIQUES
- Liquid chromatography (analytical and preparative conditions)
- Liquid chromatography coupled to tandem mass spectroscopy. Available equipment in the group:
- Ion trap (IT) mass spectrometer Bruker Esquire 3000, coupled to a liquid cromatography system Agilent Series 1100
- Quadrupole-time of flight (Q-TOF) mass spectrometer Bruker Microtof QII, coupled to a Waters Acquity UPLC
- MALDI-TOF/TOF mass spectrometer Bruker Autoflex speed, nano chromatography system and fraction collector for MALDI plates (Proteineer fcII).
- Gel electrophoresis (inmunoblotting, in-gel digestion and mass spectrometry analysis) and capillary electrophoresis
- Immunoenzymatic techniques (Western-Blot, ELISA, ELLA)
- Spectrometric and fluorimetric techniques
- Quantitative real time PCR (gene expression in cellular and animal models)
- Flow cytometry
- Sensory analysis
- Blood pressure measurement in animal models (indirect method, tail cuff)
CELLS AND TISSUES
- Cell viability and cycle studies. Apoptosis assays
- Biomarkers of oxidative stress, allergy, inflammation and carcinogenesis
- Studies on immune response against food allergens
- Vascular reactivity studies
- Cell lines:
- Mouse macrophages RAW264.7
- Rat intestine IEC-6 cells
- Hamster lung V79 cells
- Human colon (HT-29 y Caco2), prostate (PC-3), lung (A-549), stomach (AGS), liver (HepG2), and ovary (Caov-3) cancer cells
- Primary cell cultures:
- Human peripheral blood mononuclear cells (PBMC) and derived subtypes (polymorphonuclear cells, monocytes, etc)
- Spleen, mesenteric lymph nodes, Peyer´s patches and peritoneal macrophage cell cultures from Balb/C and C3H/HeOuJ mice
- Aorta vascular smooth muscle and fibroblast cell cultures from rat
ANIMAL MODELS
- Mouse models of oral and intraperitoneal sensitization to food allergens (egg, milk, peanut) using BALB/c and C3H/HeOuJ strains
- Mouse models of anaphylaxis against food allergens (egg, milk, peanut) with BALB/c and C3H/HeOuJ strains
- Rat models of obesity, type II diabetes and metabolic syndrome induced by the diet
- Rat models of toxicity by heavy metals to study cardiometabolic complications linked to oxidative stress
- Wistar, Sprague-Dawley (SD), spontaneously hypertensive (SHR), Wistar-Kyoto (WKY) and genetically obese (Zucker) rats, to evaluate functional foods and ingredients with beneficial effects on cardiometabolic health